Medical Director

Alissandro "Andy" Castillo, MD, MBA

Dr. Andy Castillo is a board-certified physician, and the owner and Medical Director of De Novo Aesthetics and M.D. Laser Studio. His education includes a BS from the University of Maryland, an MD from Wake Forest University, and an MBA from Duke University Fuqua School of Business. While serving in the United States Army as a flight surgeon he also successfully completed a plastic and reconstructive surgery research fellowship at prestigious Johns Hopkins. WIth his unique combination of procedural protocol and technique, Dr. Castillo has trained countless other nurses and physicians how to administer Botox. He has personally treated or directly supervised nearly 20,000 Botox treatments for wrinkle revision, hyperhidrosis, migraines, and TMJ,

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Medical Director

Rebecca Appleton Castillo, MD, MS

Dr. Appleton, the wife of Dr. Castillo, is a board-certified physician and Co-Founder and Co-Medical Director for De Novo Aesthetics. She earned a BS from Duke University and an MS from University of Maryland. She then completed her Doctor of Medicine degree at the University of Maryland Medical School. Dr. Appleton and Dr. Castillo have raised 6 children and owned and operated 5 successful medical practices. In addition to overseeing operations for De Novo, she and Dr. Castillo offer CME courses to fellow physicians and nurses. Dr. Appleton directly reviews the medical history of every potential Botox Express patient.



Certified Injection Specialist ~ Charlotte

Shanti Coates, RN, BSN

Born and raised in Florida, Shanti earned her BSN at University of Florida. She and her husband moved to NC to take advantage of its proximity to both the ocean and the mountains. Shanti enjoys the outdoors and especially loves yoga. Her hobbies include hiking, painting, traveling, and playing with her plump pug, Tank! You can find Shanti at our Charlotte location, and online @aestheticsnurseshanti.

Certified Injection Specialist ~ Lake Norman

Cara Wicker, RN, BSN

Cara has been a nurse since 2010 in addition to being a mother of three. She is a Certified Botox Injection Specialist and also serves as HIPAA Coordinator for De Novo, helping to ensure that all processes are in place to protect patient privacy. Cara manages our Instagram account: be sure to follow us @denovoaestheticss and follow Cara @aestheticsnursecara!

Certified Injection Specialist ~ Lake Norman


Allison Robinson, RN, BSN

Allison earned her BSN from Queens College in 1996. Born and raised Charlotte, Allison and her three kids enjoy watching and playing sports and consider themselves true blue NC Tarheels fans! She became a Certified Botox Injection Specialist for DeNovo in 2017 joining our Lake Norman team.


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Certified Injection Specialist ~ Lake Norman

Brooke Foster Cathey BSN, RN

Brooke is the latest addition to the De Novo Aesthetics team. You can find Brooke in our Carmel Village location and our South Boulevard location. She is also available for concierge appointments and events. We're so happy to have this lovely young bride as our most recent Certified Injection Specialist!


MD = Doctor of Medicine
MBA = Masters of Business Administration
MS = Master of Science
RN = Registered Nurse
BSN = Bachelor of Science in Nursing

NP = Nurse Practioner


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Office Manager

Lauren Frogge

After graduating from the Aveda Institute and managing salons for the last few years, Lauren joined  De Novo Aesthetics. As Office Manager, Lauren is the friendly voice at the other end of phone calls and texts scheduling appointments and answering questions.  She keeps all the locations supplied and staffed and helps coordinate promotions and events. If you are interested in co-promotions or an event with De Novo contact Lauren directly at

For full-service medical cosmetic services, check out M.D. Laser Studio.

M.D.Laser Studio

Mission Statement

We will bring clinical knowledge, skills, and experience in medical cosmetic treatments to enhance and maintain the natural youthful appearance of our clients in the comfort and security of their home or one of our convenient locations.